Volume 4, Issue 3, September 2019, Page: 33-38
Monitoring the Activity of Feed Enzymes in Vitro and Their Activity in the System that Modulates the GIT
Valeriy Sergeevich Kryukov, LLC Vetpharmstandart, Moscow, Russian Federation
Ilona Vyacheslavovna Glebova, Kursk State Agricultural Academy, Kursk, Russian Federation
Sergej Vladimirovich Zinoviev, FSC "VNITIP" RAS, Sergiev Posad, Moscow, Russian Federation
Received: Jun. 26, 2019;       Accepted: Sep. 12, 2019;       Published: Oct. 23, 2019
DOI: 10.11648/j.eeb.20190403.12      View  59      Downloads  9
Abstract
24 feed enzyme endo-1,4-glucanase (glucanase) and endo-1,4-xylanase (xylanase) have been described. Their activity was studied under in vitro conditions simulating the gastrointestinal tract of the poultry. A decrease in the temperature of the medium from 50°C to 38°C reduced the activity of all enzymes. Fekord 2004-S, Agrocell Plus, Agrocell, Rovabio max AP, Xibeten-Gel, Axtra XAP 101 TPT, Endofeed DC at pH 3, 38°C retained the highest initial xylanase activity: 81, 80, 70, 70, 67, 67, 65% respectively. The minimum stability at pH 3 was established for Sunzyme, Hostazym С-100, Ronozyme VP, Natugrain TS and Ronozyme WX: 46, 44, 42, 40, and 38%, respectively. At pH 7, 38°C it was better activity than the average for all preparations showed С Axtra XAP 101 TPT, Fekord-2004-C, Econase XT-25, Agrocell: 52, 47, 47, 45%; minimal activity was shown by Rovabio Exel AR, Cellulase, Cellolux F, Sunzaim: 4, 5, 7, and 8%, respectively. An outstanding high activity of cellulаse at pH 3, 38°C was found in Fekord 2004-C - 90%, above the average value it was in Econase XT 25, Acra XB 201 TPT, Agroxil Plus and Vilzim: 63, 62, 60 and 58%. The minimum activity at pH 3, 38°С was found in Rovabio Exel, Rovabio Max AR, Cellolux F and Xybeten-Cel: 20, 23, 23 and 25%. At pH 7, 38°С, the maximum activity of most enzymes remained within 30%, excluding Hostazym S-100 - 52%. The minimum glucanase activity at pH 7 was shown by Rovabio Max AP - 4%, Xybeten-Cel - 5%, Endofeed DC - 6%, and Rovabio Exel AP -6%. The average activity of cellulase for all studied enzymes was 19.1% of that determined at pH 5, and 50C. Under all experimental conditions, the activity of xylanases of the studied enzymes was mainly better than glucanases. This indicates greater cellulases stability. It has been substantiated that the activity indicated by the manufacturer serves only for labeling the product and does not reflect its action in the body. Used matrix values of enzymes are intended for commercial purposes and weakly reflect the real effect of enzyme.
Keywords
Feed Enzymes, Activity Enzymes, Choice Feed Enzymes, Matrix Values Enzymes
To cite this article
Valeriy Sergeevich Kryukov, Ilona Vyacheslavovna Glebova, Sergej Vladimirovich Zinoviev, Monitoring the Activity of Feed Enzymes in Vitro and Their Activity in the System that Modulates the GIT, Ecology and Evolutionary Biology. Vol. 4, No. 3, 2019, pp. 33-38. doi: 10.11648/j.eeb.20190403.12
Copyright
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Reference
[1]
Inborr J. and Grönlund A. 1993. Stability of feed enzymes in physiological conditions assayed by in vitro methods. Agric. Sci. Finl. Vol. 2. p. 125-132.
[2]
Kryukov V. S., Glebova I. V., Antipov A. A. Evaluation of fitases action in gastrointestinal tract and the use of fitase preparations in animal feeding: a review. Problems of Productive Animal Biology, 2019. No2. P. 19-43.
[3]
Volchok A. A., Korotkova O. G., Kondratyev E. G., Kryukov V. S., Sinitsyna O. A., Sinitsyn A. P., Shashkov I. A. The activity of glucanases and xylanases of feed enzyme preparations in the gastrointestinal tract of poultry. J. "Poultry farming." 2018. No04. S. 39-45.
[4]
Vasquez M. V. and Glitsoe V. Phytase Unit Myth! 2012. https://www.dsm.com/content/dam/dsm/anh/en_US/documents/2012_Phytase_unit_myths.pdf.
[5]
Menezes-Blackburn D., Gabler S., Greiner R. Performance of Seven Commercial Phytases in an in Vitro Simulation of Poultry Digestive Tract. J. Agric. Food Chem. 2015. Vol. 63. P. 6142–6149.
[6]
Masey O’Neill H. V., Smith J. A. and Bedford M. R. Multi- carbohydrase enzymes for non-ruminants. Asian-Australas. J. Anim. Sci. 2014. Vol. 27. p. 290-301.
[7]
Bedford M., Schulze H. 1998. Exogenous enzymes for pigs and poultry. Nutr Res Rev. 1996. Vol. 11. P. 91–114.
[8]
Kalmendal, R. and R. Tauson. Effects of a xylanase and protease, individually or in combination, and an ionophore coccidiostat on performance, nutrient utilization, and intestinal morphology in broiler chickens fed a wheat-soybean mealbased diet. Poult. Sci. 2012. Vol. 91. p. 1387-1393.
[9]
Zuo J., Ling B., Long L., Li T., Lahaye L., Yang C., Feng D. Effect of dietary supplementation with protease on growth performance, nutrient digestibility, intestinal morphology, digestive enzymes and gene expression of weaned piglets. J. Animal Nutrition. 2015. Vol. 1. p. 276-282.
[10]
Ingelmann C.-J., Witzig M., Möhring J., Schollenberger M., Kühn I., Rodehutscord M. Effect of supplemental phytase and xylanase in wheat-based diets on prececal phosphorus digestibility and phytate degradation in young turkeys. Poultry Science. 2018. Vol. 97. p. 2011–2020.
[11]
Roofchaei A., Rezaeipour V., Vatandour S., Zaefarian F. Influence of dietary carbohydrases, individually or in combination with phytase or an acidifier, on performance, gut morphology and microbial population in broiler chickens fed a wheat-based diet. Animal Nutrition. 2019. Vol. 5. p. 63-67.
[12]
Kalmendal R. and Tauson R Effects of a xylanase and protease, individually or in combination, and an ionophore coccidiostat on performance, nutrient utilization, and intestinal morphology in broiler chickens fed a wheat-soybean meal-based diet. Poultry Sci. 2012. Vol. 91. p. 1387–1393.
[13]
Alagawany M., Elnesr Sh. S., Farag M. R. The role of exogenous enzymes in promoting growth and improving nutrient digestibility in poultry. Iran J. Vet. Res. 2018 Vol. 19. p. 157–164.
[14]
Torres-Pitarch A., Manzanilla E. G., Gardiner G. E., O’Doherty J. V. Lawlor P. G. Systematic review and meta-analysis of the effect of feed enzymes on growth and nutrient digestibility in grow-finisher pigs: Effect of enzyme type and cereal source. Animal Feed Science and Technology. 2019. Vol.251. p. 153-165.
[15]
Noy Y. and Sklan D. Digestion and Absorption in the Young Chick. Poultry Sci. 1995. Vol. 74. p. 366-373.
[16]
Bedford, M. R. The effect of enzymes on digestion. J. Appl. Poult. Res. 1996. Vol. 5. P. 370-378.
Browse journals by subject