Volume 4, Issue 4, December 2019, Page: 55-59
Cross-species Amplification of Scallop Microsatellites in the Family Pectinidae
Min Hui, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China
Received: Sep. 3, 2019;       Accepted: Sep. 24, 2019;       Published: Nov. 7, 2019
DOI: 10.11648/j.eeb.20190404.12      View  42      Downloads  8
Abstract
In the present study, 103 microsatellite markers were selected for cross-species amplification in four marine economic scallop species (Argopecten irradians, Chlamys farreri, Chlamys nobilis, and Mizuhopecten yessoensis) belonging to the family Pectinidae. As a result, in the 72 markers originated from C. farreri, 12 showed amplification products in C. nobilis, 12 in A. irradians and 11 in M. yessoensis. In the 12 markers developed from M. yessoensis, four markers could be amplified successfully in A. irradians, three in C. farreri, and one in C. nobilis. In the nine markers isolated from C. nobilis, two markers could be cross-amplified in A. irradians and one in M. yessoensis, but no marker was likely to be useful in C. farreri. In the nine markers of A. irradians, one marker was potentially workable for C. farreri, one in M. yessoensis, but none in C. nobilis. When the microsatellites were cross-species amplified, most of the PCR products showed low yield and ambiguous bands, while the numbers of alleles also decreased. Finally, three markers (CFMS016, CFCD131 and CFE04) were revealed to be successfully transferred among the four species providing candidate markers for ecological study of scallops, while most of the other markers were unique for one species with poor cross-species amplification, which might be useful for species identification.
Keywords
Cross-amplification, Genetic Marker, Scallop, Species Identification, Simple Sequence Repeats
To cite this article
Min Hui, Cross-species Amplification of Scallop Microsatellites in the Family Pectinidae, Ecology and Evolutionary Biology. Vol. 4, No. 4, 2019, pp. 55-59. doi: 10.11648/j.eeb.20190404.12
Copyright
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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